A three-judge committee published the ruling Saturday, saying petitioners offered significant cause to think there are "grave statutory and constitutional issues" with the federal order.
Texas Gov. Greg Abbott, who backed the lawsuit, announced an emergency hearing will be held "soon," adding in a statement on Twitter that "we will have our day in court to strike down Biden's unconstitutional abuse of authority."
The momentary halt was endorsed by those on the Right, with Texas Attorney General Ken Paxton tweeting, "Yesterday, I sued the Biden Admin over its unlawful OSHA vax mandate. WE WON."
GONE TOO SOON: NYPD MOURNS FALLEN OFFICER AFTER FATAL CONFRONTATION, IS BIDEN'S AMERICA TO BLAME?
But White House deputy press secretary Karine Jean-Pierre said the administration is confident the mandate will hold legal weight in court.
"We are very confident that it can," Jean-Pierre said at a Friday press briefing. "As for the legal side of this, let me be crystal clear to avoid what appears to be possible misinformation or disinformation around the emergency temporary standard being a vaccine mandate. That would be, on its face, incorrect as has been explicit for months. It is a standard for a safe workplace to either comply with weekly testing or to be vaccinated."
GONE TOO SOON: NYPD MOURNS FALLEN OFFICER AFTER FATAL CONFRONTATION, IS BIDEN'S AMERICA TO BLAME?
Just hours after the White House issued its new vaccine mandate this week, more than two dozen Republican-led states announced lawsuits questioning the measure.
RE-SHAPING THE FUTURE: TENNESSEE GOV. BILL LEE UNVEILS BOLD PLAN FOR "EDUCATION FREEDOM"
The Biden administration announced Thursday it was arranging a deadline of Jan. 4 for large companies, designated for those with more than 100 employees, to start demanding vaccination against COVID-19 or enforce weekly testing.
WATCH: TULSI GABBARD ADDRESSES WHO REALLY INFLUENCES POLICY, AND IT'S NOT BIDEN...
Biden issued a statement Thursday morning defending the move. "For our country, the choice is simple: get more people vaccinated, or prolong this pandemic and its impact on our country," the president said. "The virus will not go away by itself, or because we wish it away: we have to act. Vaccination is the single best pathway out of this pandemic."
DIPLOMATIC RIFT DEEPENS: NETANYAHU CANCELS TALKS WTH BIDEN AFTER UN CALLS FOR CEASEFIRE
Biden added that he wished the requirements weren't necessary but that current vaccination rates won't get the U.S. "out of this pandemic for good." He also cited a 40% reduction in the number of unvaccinated Americans from late July, when the first mandates rolled out, to today.
WATCH: AOC DISPLAYING HER IGNORANCE ONCE AGAIN
The law was meant to be implemented by the Department of Labor's Occupational Safety and Health Administration, a move that leaves the judicial branch to determine whether it stands as an unconstitutional mandate or an enforceable component of safety standards in large workplaces.
The court gave the U.S. government until 5 p.m. Monday to respond to the stay.