The US Open organizers have announced they would respect the government's rules on non-citizens being completely vaccinated in order to enter the nation, ruling out Djokovic.
Djokovic's anti-vaccine stance has been well-documented. In January 2022, he was deported from Australia bbefore the Open in Melbourne over his refusal to get vaccinated.
Following the drama Down Under, Djokovic, 35, announced that he would not get the jab only to compete in tournaments.
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The former world number one explained to the BBC in February that he had vaccines as a child and that he was not "against vaccinations."
He went on: "I've always supported the freedom to choose what you put in your body."
Djokovic was hopeful of finishing in Flushing Meadows next month yet won't risk a repeat of the deportation furor he went through in Australia.
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The Serbian superstar has won the US Open on three occurrences.
He was named on the entry list for the final grand slam of the year on Wednesday, yet that is routine, instead of an indication that he will be allowed to play.
The list is made up of the highest ranking players in the world. Djokovic is presently ranked seventh. He is only one significant win away from tying with fellow legend Rafa Nadal for 22 major titles.
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Djokovic announced when questioned regarding playing at the US Open: "I'm not going to go to America if I don't have permission, so the Australian saga for me was not pleasant at all."
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"People still think I forced my way to Australia and tried to come in with no papers, permission or exemption - it is not true."
"That was proven in the court cases, so I would never go into a country where I didn't have permission to travel."
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"I would love to come back to Australia. I love Australia, I had my best Grand Slam results in that country."
"Hopefully I can be there in January because I want to be there, and I also want to be in New York."
"I want to be in America and everywhere I can possibly play."
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After his Wimbledon victory, Djokovic explained that he was on "vacation."
He continued: "Whether or not I'm playing any tournament soon, I'll definitely be resting for the next couple weeks because it has been quite an exhausting and demanding period for me the last few months."