The tongue-in-cheek petition compares Bezos, who is supposed to launch to the edge of space on his Blue Origin rocket on July 20, to superman villain Lex Luthor.
He will fly with his brother and two others, including the winner of a £20 million pound auction, on the first crewed flight of the autonomous New Shepherd rocket.
Titled 'Petition To Not Allow Jeff Bezos Re-Entry To Earth,' the change.org page asks people to sign to keep him out, continuing 'The fate of humanity is in your hands.'
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The petition was started five days ago and is addressed to Blue Origin, the rocket launch company founded by Jeff Bezos in 2000 and operator of the expected flight.
It has been signed by more than 6,800 people at the time of writing but has new signatures arriving every few minutes, with some recommending that he 'takes Elon [Musk]' with him.
'Jeff Bezos is actually Lex Luthor, disguised as the supposed owner of a super successful online retail store,' the petition description says, adding he is 'actually an evil overlord hellbent on global domination.'
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'He's also in bed with the flat earth deniers,' the petition jokingly insists, continuing 'it's the only way they'll allow him to leave the atmosphere.'
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The creator of the petition, Jose Ortiz, suggested the upcoming spaceflight was a perfect opportunity to rid the world of the man he describes as an 'evil overlord' and urges Blue Origin to stop the rocket returning to Earth.
He further jokingly says governments stand by and watch as he takes over the world.
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Ortiz pokes fun at Bezos, declaring he is in cahoots with ‘the Epsteins and the Knights Templar,’ as well as the flat Earth deniers.
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He says keeping him in space is necessary before this Bezos-led cabal 'enable the 5G microchips and perform a mass takeover'.
Bezos and the three other space tourists will venture up to 62 miles above the Earth's surface on the New Shepherd rocket.
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This is the point thought to be the boundary between Earth's atmosphere and outer space, although NASA recognises 50 miles above the surface as the boundary of space.
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They will launch on July 20, the 52nd anniversary of the Apollo 11 moon landing, traveling at over 2,000 mph to get into space.
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After its moments in orbit, providing a few minutes of weightlessness, the innovative vehicle is supposed to float back on parachutes and land upright.
New Shepard - titled after first American in space Alan Shepard - flies by automation, is 59ft high, and can carry up to six passengers.
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