Biden used his speech to attack the "gun lobby," and advocate for "common sense gun laws," saying "As a nation we have to ask when in God's name we're gonna stand up to the gun lobby. When in God's name, when we all know in our gut what needs to be done."
Soon after the speech ended, Carlson slammed Biden for "desecrating the memory" of the recently deceased children and of "dividing the country in a moment of deep pain."
"President of the United States. Frail, confused, bitterly partisan, desecrating the lives of recently murdered children with tired talking points of the Democratic Party," Carlson said.
WATCH: UFC'S DANA WHITE GIVES REPORTER A 1ST AMENDMENT LESSON
"Dividing the country in a moment of deep pain rather than uniting. His voice rising, amplified only as he repeats the talking points he repeated for only 35 years in the United States Senate. Partisan politics being the only thing that animates him. Unfit for leadership of this country," he added.
WATCH: SOPRANOS STAR'S SHOW GETS INTERRUPTED BY A CLIMATE NUT
In his speech, Biden condemned the gun shop that sold 18-year-old suspect Salvador Ramos and other young adults weapons, saying "what in God's name do you need an assault weapon for except to kill someone?"
BUSTED: LEADER OF MASSIVE HUMAN TRAFFICKING RING FESSES UP SHOCKING DETAILS
"It's just sick. The gun manufacturers have spent two decades aggressively marketing assault weapons which make them the most and largest profit. For God's sake we have to have the courage to stand up to the industry.
"Here's what else I know. Most Americans support common sense laws, common sense gun laws," he said.
HEZBOLLAH OPERATIVE CAUGHT SNEAKING ACROSS U.S.-MEXICO BORDER
"Why do we keep letting this happen? Where in God's name is our backbone to have the courage to deal with and stand up to the lobbies," he later asked.
Biden persisted on to issue a call for prayer for the families of those killed in Robb Elementary School.
"Our prayer tonight, to those parents lying in bed trying to figure out 'will I be able to sleep again? 'What do I say to my other children? What happens tomorrow?'
May God bless the loss of innocent life on this sad day. May the Lord be near the brokenhearted and save those crushed in spirit. They're going to need a lot of help and a lot of our prayers. God love you," he concluded.