Although, expectedly, Trump has said he will still attend the debate for a chance to talk about Hunter Biden's email scandal.
The Presidential Commission on Debates announced each candidate's microphone at the debate in Nashville, Tennessee, would be silenced to enable the other to perform two minutes of opening statements at the opening of each 15-minute segment of the debate.
Both microphones will be turned on to allow a back-and-forth afterward.
On Monday, the Trump campaign sounded objections to the change, though they said the leader would attend the Thursday night event - one of his last opportunities to reach a large prime-time audience before voting ends on November 3.
The new mute feature was added after the president repeatedly spoke over both his rival and the moderator at last month's debate in breaking one of its agreed-upon rules.
'I will participate, but it’s very unfair that they changed the topics and it is very unfair that again we have an anchor who is totally biased.' Trump said.
That point was reiterated by campaign manager Bill Stephen. He stated that ’President Trump is committed to debating Joe Biden regardless of last-minute rule changes from the biased commission in their latest attempt to provide advantage to their favored candidate.
'Why did Biden allow his son Hunter to sell access to him while he was vice president, and why were there Chinese payment arrangements for Joe himself worked out by Hunter and his sketchy partners? If the media won’t ask Joe Biden these questions, the President will, and there will be no escape for Biden.'
The Biden campaign did not instantly react to a request for comment. 'As usual, the president is more concerned with the rules of a debate than he is with getting a nation in a crisis the help it needs,' said Biden spokesman TJ Ducklo.
The number of Americans who voted early stood at 30.2 million on Monday, according to the University of Florida's United States Elections Project. That number factors more than one-fifth of all the votes cast in the 2016 election.
In Florida, where no less than 2.5 million have already voted by mail, residents rushed for the first day of early in-person voting.
Registered Republican Antonio Sanchez, an architect who landed in the United States from Communist Cuba, said he encourages Trump because he 'stands for freedom' and against socialism.
'My two daughters are doctors,' said Sanchez, 59. 'I don't think this could have happened anyplace other than America.'